高效液相色谱测定番茄制品中的三种功能性脂肪酸

邓俊琳; 陈建; 朱永清; 夏陈; 张盈娇; 邓海云; 李娟

doi:10.13386/j.issn1002-0306.2018.21.041

高效液相色谱测定番茄制品中的三种功能性脂肪酸

Determination of Three Functional Fatty Acids in Tomato Products by High Performance Liquid Chromatography

摘要

摘要: 通过高效液相色谱-质谱技术和高效液相色谱技术对番茄制品中9-羟基-10(E),12(Z)-十八碳二烯酸、9-羰基-10(E),12(Z)-十八碳二烯酸和9-羰基-10(E),12(E)-十八碳二烯酸三种功能性脂肪酸进行定性定量分析。结果表明,在色谱柱为Eclipse XDB-C18(4.6 mm×250 mm,5 μm),流动相为0.005%的三氟乙酸水溶液和0.005%的三氟乙酸乙腈溶液,检测波长为234、280 nm的条件下,番茄制品中的三种脂肪酸在20 min内得到较好分离,重复性好(RSD ≤ 2.13%),精密度高(RSD ≤ 0.70%),稳定性好(RSD ≤ 0.98%),加标回收结果准确可靠(平均回收率分别为99.78%、99.70%、99.39%,RSD ≤ 3.38%)。该色谱方法快速、准确、可靠;测试结果表明,新鲜番茄中不含有这三种功能性脂肪酸,其仅存在于番茄制品中,且加工工艺对其含量影响较大;此外9-羟基-10(E),12(Z)-十八碳二烯酸为首次在番茄制品中发现。

Abstract: The Three functional fatty acids of 9-hydroxy-10E,12Z-octadecadienoic acid,9-oxo-10E,12Z-octadecadienoic acid and 9-oxo-10E,12E-octadecadienoic acid in tomato products were identified and analyzed by high performance liquid chromatography-mass spectrometry and high performance liquid chromatography. The column was Eclipse XDB-C₁₈(4.6 mm×250 mm,5 μm)and the mobile phase were made up of 0.005% trifluoroacetic acid water and 0.005% trifluoroacetic acid acetonitrile. The detection wavelength was set to 234,280 nm. The results showed that the three fatty acids were well separated within 20 min,with good repeatability(RSD ≤ 2.13%),high precision(RSD ≤ 0.70%),good stability(RSD ≤ 0.98%)and reliable recovery(RSD ≤ 3.38%). A rapid and accurate method for the determination of 9-hydroxy-10E,12Z-octadecadienoic acid,9-oxo-10E,12Z-octadecadienoic acid and 9-oxo-10E,12E-octadecadienoic acid in tomato products was established. The results showed that fresh tomato did not contain these three kinds of functional fatty acids,which only existed in tomato products,and the processing had the greater impact on the content. Furthermore,the 9-hydroxy-10E,12Z-octadecadienoic acid was discovered for first time in tomato products.