响应面法优化热回流提取樟树叶中木脂素工艺

袁列江; 伍平香

doi:10.13386/j.issn1002-0306.2018.21.027

响应面法优化热回流提取樟树叶中木脂素工艺

Optimization of Hot Reflux Extraction Process of Lignans in Cinnamomum camphora Leaves by Response Surface Methodology

摘要

摘要: 利用响应面法优化樟树叶中木脂素的热回流提取工艺。通过单因素实验考察乙醇浓度、料液比、回流次数以及提取温度对总木脂素提取量的影响。在单因素试验基础上,采用Box-Behnken中心组合方法设计试验优化提取工艺。结果表明,最佳提取工艺条件为:乙醇浓度87%、料液比1:11 g/mL、回流3次、提取温度70℃,此条件下木脂素实际提取量为(43.39±4.91)mg/g,与理论预测值44.27 mg/g的偏差较小,即实际值与预测值之间的拟合度较好,说明响应面法对樟树叶中木脂素热回流提取条件进行优化的可行性。

Abstract: Response surface analysis was proposed to optimize the heat reflux extraction process of lignans fromthe leaves of Cinnamomum camphora. The effects of ethanol concentration,solid-liquid ratio,time,reflux times and temperature on lignans yield were investigated by single factor experiments. Based on the single factor level determination,the Box-Behnken center combination method was applied to determine the optimization of the extraction. Results showed that the optimum extraction condition were as follows:Ethanol concentration 87%,solid-liquid ratio 1:11 g/mL,reflux times 3,the temperature 70℃. Under the optimizatiom conditions,the experiment yield of lignans was(43.39±4.91) mg/g,which had less cleviation with the predicted value(44.27 mg/g). The results demonstrated that it was practicable to optimize the heat reflux extraction process using response surface methodology.