去除脱氧雪腐镰刀菌烯醇乳酸菌的筛选及其作用条件

胡杉杉; 翟瑶瑶; 陆兆新; 别小妹; 赵海珍; 张充; 吕凤霞

doi:10.13386/j.issn1002-0306.2018.21.021

去除脱氧雪腐镰刀菌烯醇乳酸菌的筛选及其作用条件

Screening of Lactic Acid Bacteria for Removal of Deoxynivalenol and Its Effect Conditions

摘要

摘要: 为了探讨乳酸菌对脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)的脱毒效果,本文利用腌鱼、泡菜、酸马奶中分离得到的乳酸菌,采用共培养的方式,旨在筛选出一株能够高效去除DON的乳酸菌,并对菌体浓度、反应温度、pH进行了研究,同时测定了该乳酸菌对DON的耐受能力。结果表明,从150多株乳酸菌中筛选得到了一株对DON有较高去除能力的副干酪乳杆菌,当菌体浓度达到10¹⁰ CFU/mL以上,反应温度为37℃,pH为7时,去除率为40.4%。且副干酪乳杆菌对DON有一定的耐受能力,中低浓度(0~50 μg/mL)的DON几乎不影响乳酸菌的生长,其为应用于饲料以及谷物中霉菌毒素的脱毒提供了依据。

Abstract: To investigate the detoxification effect of lactic acid bacteria on deoxynivalenol,using lactic acid bacteria isolated from salted fish,pickled cabbage and koumiss by co culture in order to select a lactic acid bacteria capable of efficiently removing deoxynivalenol,and the bacteria concentration,reaction temperature and pH. Effect of conditions on the lactic acid bacteria tolerance to DON was studied. The results showed that a strain of Lactobacillus paracasei with high removal ability of DON was obtained from more than 150 strains of lactic acid bacteria. When the concentration of bacteria was above 10¹⁰ CFU/mL,the reaction temperature was 37℃,and pH was 7,the removal rate was 40.4%. Lactobacillus paracasei had some tolerance to DON. Low concentration(0~50 μg/mL)of DON almost had no effect on the growth of lactic acid bacteria. It would provide a basis for its application in the feed and the detoxification of mycotoxins in cereals.