Abstract: Using soy protein and milk protein as the main base,the production formula of the protein probiotic yogurt was determined and nutritional analysis was carried out. Results showed that soy protein:whey protein(weight ratio)=1:2,lactose content 1% is the best formula. Compared with market yogurt,the protein content of homemade dual protein probiotic yogurt increased 22.8%,fat content decreased 37.3%,essential amino acid content increased 47.5%,protein digestibility corrected amino acid score(PDCAAS)increased over 70%. Dual protein probiotic yogurt was a kind of new nutritious and healthy food,it would help to improve nutrition and improve the level of human health.