庆阳红富士苹果中多酚类成分组成及含量分析

Analysis of Compostion and Content of Polyphenols in Qingyang Red Fuji Apple

摘要: 为分析庆阳各县区8个示范果园的红富士苹果中多酚组成。采用微波-超声-纤维素酶辅助法提取苹果中多酚,福林酚比色法和高效液相色谱法(High performance liquid chromatography,HPLC)测定总酚组成及含量。结果表明,各鲜果中总酚含量为0.723~1.088 mg/g,主要包括绿原酸、对香豆酸、儿茶素、表儿茶素、芦丁、根皮苷这6种多酚,其中绿原酸、对香豆酸和芦丁含量较高,分别为0.106~0.154、0.157~0.256和0.002~0.078 mg/g。庆阳苹果多酚组分多且含量高,营养丰富,食用和药用价值良好。

Abstract: To analyze the polyphenol composition of red Fuji apple in eight demonstrative orchard of Qingyang county. The polyphenols of apple were extracted by microwave-ultrasonic-cellulose assisted method, the composition and content of total phenol were determined by Flin-Ciocalteu and HPLC.The results showed that the total phenol content of fresh apples was 0.723~1.088 mg/g, which mainly included six kinds of polyphenols, such as chlorogenic acid, p-coumaric acid, catechin, epicatechin, rutin and phloridzin.Among them, chlorogenic acid, p-coumaric acid and rutin were relatively higher, which were 0.106~0.154, 0.157~0.256 and 0.002~0.078 mg/g respectively.The polyphenols from Qingyang apple had a high content of polyphenols, rich nutrition and good edible and medicinal value.