离子液体结合微波提取石上柏中穗花杉双黄酮工艺优化

王刚; 黎丹; 蒋奉秦; 曾娣菊; 谢显语; 何宇岐

doi:10.13386/j.issn1002-0306.2018.20.024

离子液体结合微波提取石上柏中穗花杉双黄酮工艺优化

Optimization of Ionic Liquid-Assisted Microwave Extraction of Amentoflavone from Selaginella Doederleinii

摘要

摘要: 为优化离子液体结合微波提取石上柏中穗花杉双黄酮的提取工艺,并建立其含量测定方法。首先,筛选不同类型的离子液体,再通过单因素试验和正交试验分别考察离子液体浓度,微波功率,固液比,提取时间以及提取温度对穗花杉双黄酮得率的影响,并将其与乙醇微波辅助提取和乙醇回流提取两种传统提取方法做比较。结果表明,提取工艺最佳条件为:2.0 mmol/LC₆ mimPF₆溶液为提取剂,固液比为1:15(g/mL),微波功率为500 W,温度为50℃,微波提取40 min,穗花杉双黄酮得率为0.61%±0.08%。与两种传统提取方法的比较结果表明,离子液体微波提取法可显著升高石上柏中穗花杉双黄酮的提取效率,缩短提取时间(p < 0.05)。综上所述,离子液体微波辅助提取法是一种快速、高效、绿色的提取方法。

Abstract: A method of microwave assisted extraction was set up and optimized based on Ionic Liquid (IL-MAE) for the determination of amentoflavone in Selaginella doederleinii.The effect of factors on the yield of amentoflavone was investigated through the single factor and orthogonal test, including ionic liquid concentration, microwave power, solid-to-liquid ratio, extraction time and extraction temperature.The best extractive method was obtained to compare with ethanol microwave-assisted extraction and alcohol refluxing extraction on the yield of amentoflavone.The results showed that, the best conditions were as follows:2.0 mmol/LC₆ mim PF₆for extraction solvent, solid-liquid ratio of 1:15 (g/mL), microwave power of 500 W, temperature of 50℃, microwave extraction time of 40 min.The yield of amentoflavone was 0.61% ±0.08% and the extraction method significantly increase amount of target (p < 0.05), which compared with the other two methods. This proved that IL-MAE would be a fast, efficient and green method to extract amentoflavone from Selaginella doederleinii.