Abstract: Objective:A preparation process of solid beverage with hawthorn and lotus leaf as the main raw material was established. At the same time, the total flavonoids and nuciferine were selected as the main quality control indicators, and their content determination methods were established.Methods:The optimum extraction techniques of aetive ingredients of Nelumbo nucifera and Semen Raphani were determined by orthogonal test, the yield of flavonoids and raymond salt precipitation and the rate of dry paste were used as the indexes of evaluation. The contents of the total flavonoids and nuciferine in compound solid beverage were determined by UV-Vis spectrophotometry and RP-HPLC.Results:The optimum extraction process of Nelumbo nucifera was 60 times the amount of 80% ethanol at 80℃ reflux extraction twice, 2.5 h for each extraction. The optimum extraction process of Semen Raphani was 10 times water extraction for 3 times, 1.5 h for each extraction.The rutin showed good linear relationship at the range of 0.0192~0.1152 mg/mL (r=0.9996).The average recovery was 100.53% with RSD was 1.45% (n=6).The nuciferine showed good linear relationship at the range of 1.5~24.0 μg/mL (r=0.9999).The average recovery was 100.16% with RSD was 1.17% (n=6).Conclusion:The optimization of the preparation method was simple, stable and feasible. The content determination method was accurate, reliable with strong specificity and good repeatability, so it could be used as scientific basis for production and quality control.