大黄中抗氧化物质的提取工艺优化

Extraction Process Optimization of Antioxidant Substances from Rhubarb

摘要: 目的:采用正交试验优化大黄中抗氧化活性物质的提取工艺。方法:以抗氧化活性为考察指标,采用超声辅助提取大黄中的抗氧化活性物质,考察乙醇浓度、固液比和提取时间对大黄抗氧化活性提取效果的影响。通过单因素实验和正交实验对提取条件进行优化。结果:在乙醇浓度60%,固液比为1:30,提取时间为30 min的条件下,获得的提取物DPPH自由基清除率最高,达到96.02%±1.36%。结论:该提取工艺简便快捷,可为工业生产提供参考。

Abstract: Objective:To optimize the extraction technology of antioxidant substances from rhubarb by orthogonal test. Methods:The extraction technology was optimized by orthogonal design using DPPH antioxidant activity as the investigation target. Factors such as extraction time, ethanol concentration and solid-liquid ratio were evaluated and optimized by single factor and orthogonal test. Results:Results showed that:when ethanol concentration was 60%, solid-liquid ratio was 1:30 and extraction time was 30 min, the extract obtained the highest radical clearance rate (96.02%±1.36%). Conclusion:The extraction process could provide a simple and quick reference for industrial production. At the same time, it also provided the theoretical basis for the comprehensive utilization of rhubarb.