Abstract: The anthocyanins components of 11 blueberries in Yunnan Lijiang and Jilin Jingyu were determined by HPLC. The regression equation of the concentration of delphinidin-3-galactoside, delphinidin-3-glucoside, cyanidin-3-galactoside, cyanidin-3-glucoside, malvidin-3-galactoside and malvidin-3-glucoside were achieved by analysis of standard. The results showed that accuracy and feasibility of the correlation coefficients was 0.9939~0.9968, precision RSDs of 2.00%~3.73% (n=5) and the recovery rates of six anthocyanins were in the range of 98.90%~100.99%. Delphinidin-3-galactoside, cyaniding-3-galactoside, malvidin-3-galactoside were contained in all samples by the intercomparison of 6 standards in 15 samples. The average content of delphinidin-3-galactoside and malvidin-3-galactoside were 223.99 and 153.34 μg/g with 65% of the total anthocyanins. Cluster 1 showed the higher contents of delphinidin-3-galactoside was contained by Lijiang Legacy, Lijiang O'neal, Jingyu Duke and cluster 2 showed the higher content of total anthocyanins of Lijiang Northland, Jingyu Earliblue by the cluster analysis of euclidean distance. In addition, the contents of delphinidin-3-galactoside and total anthocyanins were the evaluated important factor of blueberries anthocyanins characteristics.