不同来源异戊烯基焦磷酸异构酶(IDI)和脱氧木酮糖磷酸合成酶(DXS)对重组大肠杆菌番茄红素产量的影响
Effects of Different Isopentenyl Diphosphate Isomerase (IDI) and 1-Deoxyxylulose-5-phosphate Synthase (DXS) on Lycopene Production in Engineering Escherichia coli Strains
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摘要: 为获得更多异戊烯基焦磷酸异构酶(isopentenyl diphosphate isomerase,IDI)和脱氧木酮糖磷酸合成酶(1-deoxyxylulose-5-phosphate synthase,DXS)相关功能基因资源,本研究选取来源于大肠杆菌(Escherichia coli)、枯草芽孢杆菌(Bacillus subtilis)、粘细菌(Myxococcus stipitatus)、戈壁奇球菌(Deinococcus gobiensis)的4种IDI和DXS,分别克隆到表达载体,将其与含有合成番茄红素基因的pTrc99aEBI质粒共同在大肠杆菌中进行异源表达。结果表明,来源于粘细菌的IDI和DXS可以获得最高的番茄红素产量,分别达到了10.86、7.94 mg/g DCW。协同表达经过密码子优化的IDI、DXS,番茄红素产量达到了15.26 mg/g DCW。本研究通过比较不同来源的IDI和DXS,获得了新的基因资源。Abstract: In order to obtain more relevant functional gene resources of isopentenyl diphosphate isomerase (IDI) and 1-deoxyxylulose-5-phosphate synthase (DXS), four IDI and DXS genes were cloned from different species, including Escherichia coli, Bacillus subtilis, Myxococcus stipitatus and Deinococcus gobiensis were heterologous expressed in Escherichia coli with pTrc99aEBI. Results showed that, MyxoIDI and MyxoDXS were the most efficient species for lycopene production and the yield of lycopene reached to 10.86, 7.94 mg/g DCW. The yield of lycopene reached 15.26 mg/g DCW by co-expression of the codon optimized IDI、DXS. This study described new genetic resources for lycopene production by comparing the different IDI and DXS.
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