冷藏小黄鱼优势腐败菌致腐能力评价及其对挥发性成分的影响

黄佳奇; 葛雨珺; 向迎春; 余海霞; 杨水兵; 胡亚芹

doi:10.13386/j.issn1002-0306.2018.16.041

冷藏小黄鱼优势腐败菌致腐能力评价及其对挥发性成分的影响

Evaluation of Spoilage Potential of Specific Spoilage Organisms of Refrigerated Little Yellow Croaker and Its Influence on the Volatile Compound

摘要

摘要: 为探究不同优势腐败菌(Specific spoilage organisms,SSOs)的致腐能力及其对水产品挥发性成分的影响,本文以小黄鱼为原料,通过接种优势腐败菌,测定其挥发性盐基氮值(TVB-N)、菌落总数和挥发性成分含量。结果表明,变形杆菌属(Proteus hauseri)致腐能力与希瓦氏菌属(Shewanella glacialipiscicola)相当,且强于普罗威斯登菌属(Providencia heimbachae);3种优势腐败菌作用下挥发性成分主要为醇类、酯类、烃类以及含氮含硫物,其中气味的主要贡献物质为吲哚、三甲胺、二甲基二硫和二甲基三硫,S.glacialipiscicola产生的气味物质量最大,P.hauseri次之,P.heimbachae最少;优势腐败菌的产量因子与对应的含氮含硫挥发物含量变化具有一致性,相关性研究进一步显示,吲哚、三甲胺、二甲基二硫和二甲基三硫含量与TVB-N含量呈显著相关(p<0.05)。

Abstract: To explore the spoilage potential of specific spoilage organisms and its effect on the volatile compounds,little yellow croakers which inoculated by different kinds of SSOs were measured by TVB-N values,total viable counts and contents of volatile compounds. Results showed that the spoilage potential of Proteus hauseri was equal to Shewanella glacialipiscicola,and stronger than Providencia heimbachae. The species of volatile compounds from three kinds of SSOs were generally alcohols,esters,hydrocarbons,nitrogenous and sulfurous substances,while indole,trimethylamine,dimethyl disulfide and dimethyl trisulfide were the main odor substance,and S.glacialipiscicola produced the highest quantity of the odor substances while P.hauseri followed and P.heimbachae produced least. The result of yield factors was consistent with the content of nitrogenous and sulfurous compounds. The correlation study showed that indole,trimethylamine,dimethyl disulfide and dimethyl trisulfide were significantly correlated with TVB-N values(p<0.05).