运用HSCCC法分离纯化荷叶中三种黄酮及抗氧化活性

潘汇; 蔡为荣; 谢亮亮; 张杰

doi:10.13386/j.issn1002-0306.2018.16.029

运用HSCCC法分离纯化荷叶中三种黄酮及抗氧化活性

Isolation and Purification of Three Flavonoid from Lotus Leaf by HSCCC and Antioxidant Activity Evaluation

摘要

摘要: 利用高速逆流色谱技术(HSCCC)从荷叶中分离制备高纯度的黄酮类物质,并对所制备的荷叶黄酮进行抗氧化活性研究。结果表明,以正己烷:乙酸乙酯:甲醇:水=1:6:1:6和正丁醇:甲醇:水=5:1:4作为HSCCC分离的两相溶剂体系,得到三种目标组分C₁、C₂和C₃的质量为12.4、5.9、2.1 mg,经高效液相分析检测其纯度分别为92.67%、90.43%、87.52%。通过标准品对照实验,得到三种目标组分可能为紫云英苷、异槲皮苷及槲皮素。并对目标组分的抗氧化活性进行研究,得到对羟基自由基的半数清除率(IC₅₀)分别为0.94、0.85、0.76 mg/mL,对DPPH自由基的半数清除率(IC₅₀)分别为0.39、0.28、0.06 mg/mL,对ABTS⁺自由基的半数清除率(IC₅₀)分别为0.56、0.47、0.29 mg/mL,三种目标组分均表现出良好的抗氧化活性。

Abstract: Isolation and prepare high-purity flavonoid from lotus leaves by High-speed counter-current chromatography and research antioxidant activity. Results showed that:The two-phase solvent system composed of N-hexane:ethyl acetate:methanol:water=1:6:1:6 and N-butanol:methanol:water=5:1:4.The quality of the three target components(C₁,C₂ and C₃)was obtained as 12.4,5.9 and 2.1 mg and Check the purity by HPLC was 92.67%,90.43%,87.52%. The three target components were likely to be orientin,isoquercitrin and quercetin by standard control experiment.The three target components showed good antioxidant activity,the half-value clearance(IC₅₀)of hydroxyl radical was 0.94,0.85,0.76 mg/mL,the half-value of DPPH free radical scavenging rate(IC₅₀)were 0.39,0.28,0.06 mg/mL,the ABTS⁺ free radical Half of the clearance rate(IC₅₀)were 0.56,0.47,0.29 mg/mL.