分心木中化学成分的分离与鉴定

王丹; 董红敬; 杨鹏; 王晓; 耿岩玲

doi:10.13386/j.issn1002-0306.2018.14.043

分心木中化学成分的分离与鉴定

Separation and Identification of Chemical Constituents from Diaphragma juglandis Fructus

摘要

摘要: 为研究分心木的化学成分,本研究采用大孔树脂(MCI)、反复的反相C₁₈、碱性硅胶、制备液相5种色谱方法对分心木的化学成分进行分离纯化,并通过质谱、核磁共振波谱两种光谱学数据分析鉴定其化学结构。结果表明,从分心木中分离纯化得到9个化合物,分别为:2-乙氧基胡桃醌(1)、胡桃苷A(2)、4,8-二羟基-1-四氢萘醌(3)、4-megastigmen-3,9-dione(4)、3'-O-(E-4-coumaroyl)-quinic acid(5)、二氢红花菜豆酸(6)、5'-O-(E-4-coumaroyl)-quinic acid(7)、香草酸4-O-β-D-葡萄糖苷(8)和litchiol A(9),其中化合物4,5,7,8和9为首次从核桃种属植物中分离得到。本研究对分心木的化学成分有了进一步认识,为探究其药理活性奠定基础,有利于分心木的开发和利用。

Abstract: To explore the chemical constituents of Diaphragma juglandis Fructus.,the compounds were separated and purified by repeated column chromatography on macroporous resin(MCI),reversed phase C₁₈,alkaline silica gel and preparative HPLC five methods of chromatography,^and^their chemical structures were identified by spectroscopic data including electrospray ionization mass spectrometry(ESI-MS)and nuclear magnetic resonance(NMR). The results showed that nine compounds were isolated from Diaphragma juglandis Fructus and they were 2-ethoxyjuglone(1),juglanin A(2),isosclerone(3),4-megastigmen-3,9-dione(4),3'-O-(E-4-coumaroyl)-quinic acid(5),dihydrophaseic acid(6),5'-O-(E-4-coumaroyl)-quinic acid(7),vanillic acid-4-O-β-D-glucopyranoside(8)and litchiol A(9). Compounds 4,5,7,8 and 9 were found in Juglans regia L. genus for the first time. The chemical composition of Diaphragma juglandis Fructus had been explored,which would lay the foundation for exploring its pharmacological activity,and be beneficial to the development and utilization.