Abstract: Anthocyanin from mulberry was extracted by ultrasonic assisted solvent method. According to adsorption rate and desorption rate,this paper selected good performance macroporous resin from HPD600,D101,LX1180,AB-8,DA201-C for purification,and the purified material's stability were studied. The results showed that the extraction rate of anthocyanin was 2.9 mg/g when 40% of ethanol(including 0.1% HCl)was used as extracting agent with a sample/solvent ratio 1:10,and the ultrasonic time 90 min. Among macroporous resins selected,the HPD600 was the best for the purification of anthocyanin. The sugar-removing ratio was 67.6%,the purity was 16.7%,the color value was 46.1,and the product was purple black powder. It contained about 41.2% of cornflower-3-O-glucoside. With the increasing of temperature,pH and illumination intensity,the stability of anthocyanin decreased. Anthocyanin was sensitive to Cu⁺,Cu²⁺,Fe²⁺,Fe³⁺,while Na⁺,Mg²⁺,Al³⁺ had a little influence on the stability of anthocyanins. Purified anthocyanin should be processed and stored in conditions of 25℃,low pH value,the dark and avoid contact cupric ion or iron ion.