响应面法优化玫瑰花色苷超声辅助提取工艺

Optimization of Ultrasonic-assisted Extraction Process for Anthocyanins from Rosa rugosa by Response Surface Methodology

摘要: 采用超声辅助法提取玫瑰花色苷,通过单因素实验和响应面分析,优化玫瑰花色苷的提取工艺。以花色苷得率作为评价指标,在单因素实验的基础上,选取乙醇体积分数、提取温度、pH和提取时间四个影响因素进行响应面优化。结果表明,最优提取工艺为:乙醇体积分数55%、提取温度55℃、pH3.0、提取时间80 min,此条件下玫瑰花色苷得率为(10.07±0.098)mg/g,与预测值的相对误差为0.28%,说明响应面分析结果可靠。该法比对照组的提取工艺用时短、效率高。

Abstract: Response surface methodology was employed for the ultrasonic-assisted extraction of anthocyanins from Rosa rugosa. Anthocyanin yield was selected as evaluation index to investigate anthocyanin extraction process from Rosa rugosa. Based on the single factor test,response surface methodology was used to optimize the extraction process of anthocyanins with four impact factors,such as ethanol concentration,extraction temperature,pH value,extraction time. The optimal extraction conditions of anthocyanins were ethanol concentration of 55%,extraction temperature of 55℃,pH value of 3.0,and extraction time of 80 min.The actual value of anthocyanin yield was(10.07±0.098) mg/g under these conditions,relative error of 0.28% compared with the predicted value. The process had shorter time and higher efficiency compared with the control group.