一种姬松茸多糖的纯度鉴定与单糖组成分析

房雷雷; 赵肖通; 辛慧洁; 林婷婷; 张彦青; 解军波

doi:10.13386/j.issn1002-0306.2018.13.018

一种姬松茸多糖的纯度鉴定与单糖组成分析

Purity Identification and Monosaccharide Composition Analysis of a Polysaccharide Derived from Agaricus brasiliensis

摘要

摘要: 目的:鉴定一种经分离纯化得到的姬松茸多糖的纯度,并分析其单糖组成。方法:采用苯酚-硫酸法测定姬松茸多糖总糖含量,结合凝胶过滤法、高效液相色谱-蒸发光散射检测(HPLC-ELSD)法和紫外可见吸收光谱法鉴定其纯度,运用高效液相色谱-柱前衍生化法对其单糖组成进行定性、定量分析。结果:所得姬松茸多糖样品的多糖含量为99.50%±2.5%(W/W,以葡萄糖计),且不含核酸、蛋白质等杂质,纯度较高,其单糖组成为葡萄糖、半乳糖、甘露糖(含量比分别为99.2%、0.6%、0.2%)。结论:所得姬松茸多糖是一种纯度较高、葡萄糖为主要单糖的均一多糖。

Abstract: Objective:To identify the purity and analyze the monosaccharide composition of a polysaccharide isolated and purified from Agaricus brasiliensis. Methods:The polysaccharide content of Agaricus brasiliensis polysaccharide was determined by phenol-sulfuric acid method,and its purity was identified by Sephadex Filtra,High-Performance Liquid Chromatography-Evaporative Light Scattering Detection(HPLC-ELSD)method and UV-Visible Absorption Spectroscopy. High Performance Liquid Chromatography(HPLC)-Column Derivatization method was used to determine the monosaccharide composition of the polysaccharide. Results:The purity of the obtained polysaccharide was high:its polysaccharide content was 99.50%±2.5%(W/W,calculated as glucose),and it does not contain nucleic acid,protein and other substances. The polysaccharide was mainly composed of mannose,glucose and galactose,which accounts for 0.2%,99.2% and 0.6%,respectively. Conclusion:The obtained Agaricus brasiliensis polysaccharide was a homogeneous polysaccharide with high purity and more glucose content.