Abstract:
Objective:To explore the optimal extraction conditions of mangiferin from coffee leaves. Methods:On the basis of single factor experiment,the extraction conditions of mangiferin from coffee leaves was optimized by response surface methodology,using the extraction rate of mangiferin as the response value,and the ratio of material to liquid,the volume fraction of ethanol,the extraction time and the extraction temperature were selected as independent variables. Results:The optimal conditions were determined as follows:The ratio of material to liquid was 1:15 g/mL,volume fraction of ethanol was 70%,extraction temperature was 56℃,extraction time was 76 min.Under this condition,the extraction rate of mangiferin was 0.432%,the relative error between the actual value and the theoretical value was <5%. Conclusion:The results were reasonable and reliable,which could be used as the optimum extraction technology for the mangiferin.