Abstract: The antioxidant properties and total polyphenol contents of various solvent extracts from two Hypsizygus marmoreus strains were investigated. Crude extract, hexane fraction, ethyl acetate fraction, butanol fraction and aqueous fraction of Hypsizygus marmoreus were prepared. Their antioxidant properties were studied and their total polyphenol contents were determined. Results obtained in this study showed that, as for MinZhen No. 2, the highest total antioxidant activities, DPPH radical scavenging activities and ABTS radical scavenging activities were found for hexane fraction, ethyl acetate fraction and butanol fraction, respectively. As to MinZhen No. 3, the highest total antioxidant activities, DPPH radical scavenging activities and ABTS radical scavenging activities were exhibited by ethyl acetate fraction. Correlation analysis results showed that total polyphenol contents had a relation with DPPH radical scavenging activities. Hexane fraction and ethyl acetate fraction of Hypsizygus marmoreus could be used as a source of natural antioxidant applied in food science when taking into account the combination of antioxidant properties and extraction yields.