Abstract: In this study, the correlation between the matrix and Auricularia polytricha fruiting body (Shanghai No. 1 and Huang Er 10) in Sichuan area was researched to define the enrichment ability of chromium in matrix in order to provide technical support for the formulation of chromium standard in the matrix. In the experiment, five different concentrations of 0, 1, 5, 10 and 20 mg/kg were designed by adding different concentrations of chromium nitrate in the matrix, and the seeds were planted by normal cultivation method. ICP-MS method was used to identified, the SPSS variance and regression analysis were used for data. The results showed that there was no significant difference in chromium content before and after the sterilization of the substrate. The change of chromium content in different flushes was irregular.The chrome content of the fruiting body increased with the increase of chromium content in the matrix, but the growth trend decreased with the increase of chromium content in the matrix. Two equations were obtained which were (Huang Er 10):y=0.7ln (x) +0.2221, R²=0.9129, Shanghai 1:y=1.3324ln (x) -0.9914, R²=0.9574, that could be used as a reference for the limit specification setting.