蛹虫草速溶粉对环磷酰胺致小鼠肝损伤的保护作用

邵颖; 陈安徽; 张明; 郑义; 陈宏伟; 夏振荣

doi:10.13386/j.issn1002-0306.2018.09.051

蛹虫草速溶粉对环磷酰胺致小鼠肝损伤的保护作用

Protective effect of instant powder of Cordyceps militaris on cyclophosphamide-induced hepatic injury in mice

摘要

摘要: 研究蛹虫草速溶粉对环磷酰胺诱导的小鼠肝损伤的保护作用。将昆明种小鼠随机分为正常组、模型组、阳性对照组、蛹虫草速溶粉高剂量组(80 mg/kg)、中剂量组(40 mg/kg)、低剂量组(20 mg/kg),采用腹腔注射环磷酰胺(80 mg/kg)构建小鼠肝损伤模型并灌胃给予不同剂量蛹虫草速溶粉(正常组、模型组灌胃生理盐水,阳性对照组灌胃水飞蓟宾),采用试剂盒法分别测定各组小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、白细胞介素-2(IL-2)、肿瘤坏死因子(TNF-α)及肝组织匀浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽转移酶(GSH-Px)活力及丙二醛(MDA)水平。实验结果表明,与模型组相比,蛹虫草速溶粉各剂量组小鼠血清中ALT和AST活力均显著降低(p<0.01);雄性小鼠肝组织中SOD和GSH-Px活力均显著升高(p<0.01),而MDA含量显著降低(p<0.01),而仅中、高剂量组小鼠肝组织中CAT活性极显著高于模型组(p<0.01);对于雌性小鼠而言,各剂量组小鼠肝组织中CAT活力均显著高于模型组(p<0.01)、MDA含量均显著低于模型组(p<0.01),而雌性中、高剂量组小鼠肝组织中SOD、GSH-Px活力显著高于模型组(p<0.01)。另外,除雄性低剂量组小鼠血清中TNF-α与模型组间无差异外,其他各剂量组小鼠血清中白细胞介素-2(IL-2)、肿瘤坏死因子(TNF-α)的含量均显著高于模型组(p<0.01)。说明蛹虫草速溶粉对环磷酰胺导致的小鼠肝损伤具有显著的改善作用。

Abstract: The aim of this study was to investigate the protective effect of instant powder of Cordyceps militaris on liver injury induced by cyclophosphamide in mice. Kunming mice were randomly divided into normal group, model group, positive control group, high dose group (80 mg/kg), middle dose group (40 mg/kg)and low dose group (20 mg/kg). The hepatic injury model of mice was established by intraperitoneal injection of cyclophosphamide (80 mg/kg), then the mice of normal and model group were fed with normal saline, mice of positive control group were fed with silybin and the mice of dosage groups were fed with different dosage of instant powder of Cordyceps militaris. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin-2 (IL-2), tumor necrosis factor alpha (TNF-α)of serum in mice and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px)and malondialdehyde (MDA)level of liver tissue in each group mice were determined by reagent kit method. Experimental results showed that ALT and AST activity of serum in mice of every dosage group significantly decreased (p<0.01)comparing with the model group. For male mice, the activities of SOD and GSH-Px of liver tissue in mice of every dose group were significantly increased (p<0.01), and the content of MDA was decreased significantly (p<0.01), but only the CAT activity in mice of middle and high dose groups was higher than the model group (p<0.01). For female mice, the activity of SOD and GSH-Px in mice of middleand high dosage group was higher than model group dramatically (p<0.01), the dramatic differences in activity of CAT and the content of MDA existed between the dosage groups and the model group (p<0.01). In addition, the instant powder of Cordyceps militaris could significantly increase the content of interleukin-2 (IL-2)in every group and could increase the content of TNF-α in middle and high dosage group compared with the model group (p<0.01). So the instant powder of Cordyceps militaris has protective effect on cyclophosphamide-induced hepatic injury in mice.