Abstract: In order to improve utilization of by-products, Trachinotus ovatus was randomly selected to analyze fatty acids composition using gas chromatography-flame ionization detector (GC-FID)method. Lipid in five different tissues were extracted using chloroform-methanol solvent, and it was found that lipid contents from high to low were as follows:viscera (27.12±7.96) g/100 g > bone (16.04±3.35) g/100 g ≈ head (15.88±3.09) g/100 g > skin (11.93±5.72) g/100 g > meat (5.09±2.13) g/100 g. Tissues had similar fatty acids composition, and were rich in oleic acid (24.31%~25.84%), palmitic acid (23.16%~25.00%)and linoleic acid (19.04%~19.76%). Saturated fatty acid (SFA)in viscera (35.32%±2.17%)was the highest and significantly higher than that of other tissues (p<0.05). The monounsaturated fatty acids (MUFA)in bone (34.27%±1.28%), head (34.56%±1.32%)and meat (34.34%±1.57%)were significantly higher than those in viscera (32.68%±1.64%)and skin (33.22%±1.23%). The polyunsaturated fatty acids (PUFA)between the five tissues didn't have significant differences. Skin (10.34%±1.76% and 6.04%±1.53%)was the highest in n-3 PUFA and DHA+EPA, significantly higher than other tissues (p<0.05). In viscera SFA (35.32%±2.17%)>MUFA (32.68%±1.64%)>PUFA (31.09%±3.18%);MUFA > SFA ≈ PUFA in bone, head and meat. In skin SFA (33.70%±2.22%) ≥ MUFA (33.22%±1.23%) ≥ PUFA (32.07%±3.23%). Therefore, the Trachinotus ovatus tissue lipid nutrition is rich and has the value of development and utilization.