不同pH、温度、金属离子对溶菌酶抗菌稳定性的初步研究

郭文燕; 周胜男; 余雅琦; 伍金娥; 常超

doi:10.13386/j.issn1002-0306.2018.09.008

不同pH、温度、金属离子对溶菌酶抗菌稳定性的初步研究

Antimicrobial stability of lysozyme under different pH,temperature and metal ions

摘要

摘要: 本文选择金黄色葡萄球菌(Staphylococcus aureus)与大肠杆菌O157：H7(Escherichia coli O157：H7)为受试菌,以活菌抑制率为评价指标,评价不同pH、温度、金属离子条件对溶菌酶抗菌稳定性的影响。结果表明:溶菌酶对金黄色葡萄球菌与大肠杆菌O157：H7的最小抑菌浓度分别为1.3×10^-3 g/mL与2.6×10^-3 g/mL。溶菌酶在酸性条件(pH4~6),其对两种菌均能表现很好的抑菌效果。经过4~121 ℃处理后,溶菌酶的抗菌活性不受处理温度的影响。不同金属离子对溶菌酶抗菌活性影响较大,Zn²⁺、Mn²⁺与溶菌酶具有较强的协同抑菌效应,其中Zn²⁺效果最为明显,而Mg²⁺、Ca²⁺具有一定的拮抗抑菌效应,可降低溶菌酶的抑菌效果。

Abstract: In this research, Staphylococcus aureus and Escherichia coli O157:H7 were selected as the tested bacteria. To evaluate the antibacterial stability of lysozyme (LYS)at different pH, temperatures and metal ion, the inhibitory rate of LYS against Staphylococcus aureus and Escherichia coli O157:H7 was detected. The results showed that the inhibitory effect of LYS on Staphylococcus aureus was stronger than that of Escherichia coli O157:H7, and the minimum inhibitory concentration was 1.3×10^-3 g/mL and 2.6×10^-3 g/mL, respectively. LYS had a good bacteriostatic effect on Staphylococcus aureus and Escherichia coli O157:H7 under acidic conditions, especially at pH4~6.After thermal treatments from 4~121℃, the antibacterial activity of LYS was not influenced. Different metal ions had a distinguished effects on the antibacterial activity of LYS. Zn²⁺ and Mn²⁺ had a strong synergistic effect with LYS, while Mg²⁺ and Ca²⁺had some antagonistic effect, which could reduce the antibacterial effect of LYS.