Abstract: The effects of Lactobacillus plantarum DM10 9010 on lipid levels were studied. SD rats fed with high fat diet were randomly divided into normal group,model group,positive group and experimental group.The rats were treated with sterile water,1 mg/mL aqueous solution of atorvastatin calcium,high dose(10⁹ CFU/mL)and low dose(10⁷ CFU/mL)L. plantarum DMDL 9010 suspension for 10 weeks to measure the level changes of body weight,blood lipid level(total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)),liver lipid level(TC,TG,pathological sections)and fecal lipid(cholesterol,total bile acid(TBA)). The results showed that high dose of L. plantarum DMDL 9010 could significantly decrease serum total cholesterol(23.03%),low density lipoprotein cholesterol(28.00%)and arteriosclerosis index(34.04%)(p<0.05)in hyperlipidemic rats. Liver morphology and pathological section observation showed that L. plantarum DMDL9010 could improve liver cell steatosis. In addition,high-dose L. plantarum DMDL9010(10⁹ CFU/mL)significantly reduced TC(33.20%)and TG(40.86%)(p<0.05)in the liver of high-fat rats,and increased fecal TC(31.07%)and TBA(70.18%)(p<0.05). To sum up,it showed that L. plantarum DMDL 9010 might be effective in inhibiting the accumulation of cholesterol by increas the amount of TC and TBA in the feces. Liver pathology showed that L. plantarum DMDL9010 could improve hepatic steatosis.L. plantarum DMDL 9010 could reduce TC and TG in serum and liver in a dose-dependent manner,increase the excretion of TC and TBA in the feces,thereby effectively regulate its lipid levels and provide a probiotic preparation for the prevention and treatment of hypercholesterolemia.