纳他霉素对采后蓝莓果实灰霉病(<i>Botrytis cinerea</i>)防治

周福慧; 姜爱丽; 姬亚茹; 胡文忠; 陈晨; 王馨悦

doi:10.13386/j.issn1002-0306.2018.08.047

纳他霉素对采后蓝莓果实灰霉病(Botrytis cinerea)防治

Natamycin on Botrytis cinerea of postharvest blueberry fruits prevention and treatment of natamycin on of postharvest blueberry fruit

摘要

摘要: 研究单独使用纳他霉素和与维生素C(V_C)复配后使用对蓝莓灰霉病(Botrytis cinerea)的防治效果。通过离体培养实验确定 V_C溶液、纳他霉素溶液以及纳他霉素溶液与20 mg/L V_C复配后的最低抑菌浓度;通过活体实验观察发病率、病斑直径、蛋白质含量以及与抗病性相关酶活性的变化,以确定不同溶液对蓝莓灰霉病的抑制效果。实验结果表明:单独使用纳他霉素处理和与20 mg/L V_C复配溶液复配处理对3μL 5×10⁶ CFU/mL的蓝莓灰霉病菌的最低抑菌浓度分别为1和0.5 mg/L,而单独使用V_C处理的抑菌效果不明显,说明纳他霉素与V_C复配使用可有效降低纳他霉素使用量,提升作用效果;同时纳他霉素复配溶液可有效提升蓝莓抗菌能力,以30 mg/L纳他霉素复配液处理效果最好。

Abstract: The effects of the use of natamycin and complex with Vitamin C(V_C)on the control of Botrytis cinerea of postharvest blueberry fruits were studied. The minimal inhibitory concentrationsof V_C solution,natamycin solution,natamycin and complex with 20 mg/L Vitamin C(V_C)solution were determined by in vitro experiment. Morbidity,lesion diameter,protein content,and the change of enzyme activity related to disease resistance was studied to determine the inhibitory effect of different solutions on Botrytis cinerea of postharvest blueberry fruits.The results showed that the minimum inhibitory concentrations of natamycinand natamycincombination with 20 mg/LV_C compound solutionfor 5×10⁶ CFU/mL were 1 and 0.5 mg/L,while the antibacterial effect of 20 mg/L V_C was not obvious,indicating that the usage amount of natamycin could be reduced and the anti-bacterial ability could be enhanced by mixed with 20 mg/L V_C. The best natamycin concentrationof the mixture was 30 mg/L.