平阴玫瑰花托多糖的结构特征及抗氧化活性分析

马耀宏; 马润隆; 郑岚; 杨艳; 杨俊慧; 蔡雷; 刘庆艾; 庄晓凤; 王业强

doi:10.13386/j.issn1002-0306.2018.08.012

平阴玫瑰花托多糖的结构特征及抗氧化活性分析

Structural characterization and antioxidant activity of receptacle polysaccharide from pingyin rose

摘要

摘要: 为研究玫瑰花托多糖的结构特征和生物活性,采用DEAE-52纤维素阴离子交换柱层析将玫瑰花托多糖分离纯化为3个组分。结构分析表明玫瑰花托多糖的单糖组成为葡萄糖、阿拉伯糖、半乳糖、半乳糖醛酸、鼠李糖、葡萄糖醛酸、甘露糖(24.01:14.57:11.50:9.77:5.5:1.09:1),构型主要为呋喃糖。抗氧化实验表明,玫瑰花托多糖具有较强的抗氧化活性,其清除羟基自由基和DPPH自由基的EC₅₀值分别为699.46、514.31 mg/L。通过对比分析可知,玫瑰花托多糖的结构与玫瑰花瓣多糖的结构具有相似性,而玫瑰花托多糖的抗氧化活性稍强于玫瑰花瓣多糖。因此,玫瑰花托多糖是优良的天然抗氧化剂,有进一步开发利用的价值。

Abstract: In order to study the structural characteristics and bioactivity of rose receptacle polysaccharide,the rose receptacle polysaccharide was isolated and purified into three fractions by DEAE-52 cellulose column chromatography. The structural analysis showed that rose receptacle polysaccharide was composed of glucose,arabinose,galactose,galacturonic acid,rhamnose,glucuronic acid,and mannose in a molar ratio of 24.01:14.57:11.50:9.77:5.5:1.09:1,and configuration was furanose. The results of in vitro antioxidant experiments showed that rose receptacle polysaccharide had a strong antioxidant capacity,and the EC₅₀ values of scavenging hydroxyl radical and scavenging DPPH radical were 699.46 mg/L and 514.31 mg/L,respectively. Through the comparative analysis,it could be seen that rose receptacle polysaccharide had similar structure to the rose petal polysaccharide. But the antioxidant activity of rose receptacle polysaccharide was slightly stronger than rose petal polysaccharide. Therefore,the rose receptacle polysaccharide was an excellent natural antioxidant,and it had the value of further development and utilization.