黄秋葵超微粉多糖提取工艺的优化及其抗氧化活性测定

Optimization of extraction technology and determination of antioxidant activity of polysaccharides from superfine powder of okra

摘要: 以黄秋葵为原料,制备黄秋葵超微粉,利用响应面设计法优化黄秋葵超微粉多糖的提取工艺,并测定其对DPPH自由基、超氧阴离子自由基、羟自由基的清除能力和还原力。结果表明:最优工艺条件为料液比1:100(g/mL)、微波时间2 min、超声波时间14 min、超声波功率800 W,此条件下黄秋葵超微粉多糖的得率为27.68%±0.42%。黄秋葵超微粉多糖的体外抗氧化活性在四种体系中的IC₅₀值分别是1.53、4.12、6.38、2.49 mg/mL,具有较好的抗氧化活性。

Abstract: The yield of polysaccharides in superfine powder that okra was prepared into was studied by ultrasonic-microwave assisted extraction method.The optimum process conditions were determined by response surface method.The antioxidant activity in vitro of polysaccharides extracted from superfine powder of okra was determined, including DPPH radical-scavenging, hydroxyl radical-scavenging,superoxide anion radical scavenging and reducing powers.The optimum extraction conditions were as follows:microwave time 2 min, ultrasonic time 14 min, and ultrasonic power 800 W. Under this optimized conditions, the experimental and predicted yield of polysaccharides was 27.68%±0.42%.The IC₅₀ for scavenging capacity of superfine powder of okra against DPPH·,O₂^-·,·OH and reducing power were 1.53,4.12,6.38 and 2.49 mg/mL respectively.