Abstract: In the field of probiotic properties,tolerance to high temperature,bile salt and gastric acid,antibacterial activities to harmful bacteria and resistance to different antibiotics were chosen to research on Bacillus coagulans 13002.The results showed that the survival rate of strain was 72.3% after 10 minutes under 80 ℃. Under the conditions of bile salt for 12 h and gastric acid of pH2.0 for 4 h,the survival rate were 69.8% and 40.0% respectively. The strain had certain antibacterial activities to Staphylococcus aureus and Escherichia coli,with the inhibition zone of 13.650 and 14.367 mm respectively. It also resisted penicilin,streptomycin and achromycin in different MIC which were 0.781 mg/mL,0.391 mg/mL and 0.039 mg/mL. Bacillus coagulans 13002 possessed great probiotic properties.