Abstract: Polyphenol oxidase(PPO)is a widely distributed in the nature of a metalloproteinase,it can catalyze the oxidation of o-benzenediol to o-benzodioxanone. And it is also the main reason for enzymatic browning of many fruits,vegetables and other agricultural products. In this paper,the recent advances in the extraction and purification of polyphenol oxidase from domestic and foreign were reviewed,including traditional extraction methods(such as buffer homogenization,acetone extraction,acetone powder extraction,etc.),new extraction methods(ultrasonic-assisted extraction and ultra-high pressure extraction,etc.),and also including the traditional classical separation and purification methods(such as solvent method,precipitation method),the new separation and purification methods(such as gel chromatography and ion exchange chromatography,etc.). The aim of this study is to provide reference for the research and application of polyphenol oxidase,especially for the inhibition of enzymatic browning in agricultural products.