Abstract: Using soy isolate protein and skimmed milk powder as a material,the inactive double protein Lactobacillus beverage which possessed special flavor and nutrition was studied. The formula of inactive double protein Lactobacillus beverage were optimized by single factor and orthogonal test method,and the sensory score as the index. The influence of skimmed milk powder,soy isolate protein,white granulated sugar and citric acid on sensory score were investigated. The optimum formula of inactive double protein lactobacillus beverage were as follows:skimmed milk powde 2%,soy isolate protein 1%,white granulated sugar 8% and citric acid 0.25%. The score of sensory was 97,on this condition,the drink was rich in nutrition and had right sweet and sour,unique flavor,well taste.