果胶酯酶的发酵培养基及培养条件优化

Optimization of medium composition and culture conditions for Aspergillus tubingensis production

摘要: 为提高果胶酯酶的产量,以果胶酯酶活力与生物量为指标,对塔宾曲霉CICC 2651产果胶酯酶的发酵培养基和培养条件进行了研究,通过单因素实验得到最优培养基和培养条件为:硫酸铵0.5%,果胶3%,Na₂SO₄ 0.04%,MgSO₄·7H₂O 0.04%,K₂HPO₄ 0.2%,培养温度30 ℃,初始pH为4.5,接种量5%,装液量40 mL。在优化工艺条件下,果胶酯酶活力达到1.53±0.09 U/mL,比初始条件提高了77.9%。通过发酵培养基和培养条件优化,塔宾曲霉CICC 2651发酵产果胶酯酶的能力大幅度提高。

Abstract: In order to increase the yield of pectin esterase,the medium compositon and culture conditons were studied for fermentation of Aspergillus tubingensis CICC 2651.Resulted in biomass and pectin esterase activity,the optimal medium compostion was composed of high-ester pectin 3%,ammonium sulfate 0.5%,Na₂SO₄ 0.04%,MgSO₄·7H₂O 0.04%,K₂HPO₄ 0.2%,culture temperature 30℃,initial pH4.5,inculation volume 5%,liquid volume 40 mL. Compared to the basic technical contidtions,the enzyme activity at the optimum technique reached 1.53±0.09 U/mL,and increased by 1.79-fold. Under the optimum fermentation process,the production capability of pectin esterase by Aspergillus tubingensis CICC 2651 was significantly improved.