Abstract: The purpose of this study was to investigate the effects of chitooligosaccharides-Zn²⁺ on intestinal microbiota in induced aging mice based on Miseq high-throughput sequencing analysis. In this study,the model of mice with induced aging were established with D-galactose injection. Then,the mice were randomly divided into seven groups,including the blank control,the model group,the groups treated with chitooligosaccharides,ZnSO₄,chitooligosaccharides plus ZnSO₄ and chitooligosaccharides-Zn²⁺ respectively. The results indicated that the numbers of valid sequence and OTU in different groups were ranged from 31727 to 36486 and from 255 to 376,respectively,in which the blank control showed the less OTU number,while the chitooligosaccharides and chitooligosaccharides-Zn²⁺ groups showed higher OTU numbers. At the phylum level,the abundances and rates of Firmicutes amd Bacteroidetes were significantly affected by the D-galactoseinjection,while the treatments of chitooligosaccharides and chitooligosaccharides-Zn²⁺ showed the positive roles on the above two phylum. At the family level,the abundance of Bacteroidales_S24-7_group was significantly(p<0.05)increased in the model groups,but the chitooligosaccharides-Zn²⁺ decreased its abundance,which also increased the abundances of Peptococcaccae,Bacteriodesruminocola,and Lachnospiraceae compared with the model group. Additionally,the chitooligosaccharides-Zn²⁺ significantly(p<0.05)increased the index of Ace,Chao and Shannon and decreased the index of Simpson compared with the model group. The results of cluster and PCA analysis indicated there were no significant difference(p>0.05)between the V_C and chitooligosaccharides-Zn²⁺ group,the proportion and distribution of which were close to the blank control group. In conclusion,the bacterial diversity and species abundance of intestinal microbiota in mice was significantly affected by the injection of D-galactose,while the treatment of chitooligosaccharides-Zn²⁺ showed the therapeutic effects on the intestinal microbiota in induced aging mice.