响应面法优化新疆芜菁多糖提取工艺及体外抗氧化活性研究

王伟; 杨晓君; 高蕾; 戴小华; 陈恺

doi:10.13386/j.issn1002-0306.2018.02.043

响应面法优化新疆芜菁多糖提取工艺及体外抗氧化活性研究

Optimization the extraction process of polysaccharide by response surface methodology from Brassica rapa L.and its antioxidant activity

摘要

摘要: 在单因素实验结果上,采用响应面法(RSM)的Box-Behnken design(BBD)实验对新疆芜菁多糖的提取工艺进行优化,并利用DPPH自由基清除能力和还原力评价其体外抗氧化能力。结果表明:最优的提取条件为提取温度93℃,液料比为75 mL/g,提取时间4.3 h,提取次数3次,在该最优提取条件下进行验证实验,测得的新疆芜菁多糖得率为23.72%±0.33%。此外,体外抗氧化实验表明:新疆芜菁多糖的DPPH自由基清除能力和还原力具有量效依存关系,其在两种体系中的EC₅₀值分别是8.55和2.25 mg/mL,表明新疆芜菁多糖具有较强的体外抗氧化能力,可以作为天然抗氧化剂应用于功能食品或者制药工业。

Abstract: Optimization the extraction process of polysaccharide by response surface methodology from Brassica rapa L. and its antioxidant activity were investigated by assays of DPPH free radical scavenging ability and reducing power. The optimal extraction conditions with an extraction yield of 23.72%±0.33% for BRP were obtained as follows:extraction temperature 93℃,extraction time 4.3 h,extraction times 3 and ratio of extraction solvent to raw material 75 mL/g. Furthermore,the BRP exhibited relatively higher antioxidant activity in vitro,DPPH free radical scavenging ability and reducing power of BRP were concentration dependent,the EC₅₀ were 8.55 and 2.25 mg/mL,respectively,hence it could be used as a natural antioxidant in functional foods or medicines.