富硒青钱柳多糖对α葡萄糖苷酶及HepG2细胞葡萄糖消耗的影响

Effects of selenium polysaccharide from Cyclocarya paliurus (Batal)Ijinskaja on α-glucosidase and glucose consumption in HepG2 cells

摘要: 以α-葡萄糖苷酶和HepG2细胞作为体外受体模型,研究富硒青钱柳多糖(Se-CPP)体外降血糖活性,并与青钱柳多糖(CPP)、CPP+硒酵母、CPP+亚硒酸钠复配物比较。结果表明,Se-CPP对α-葡萄糖苷酶活性具有良好的抑制效果且呈现明显的剂量依赖性,其半抑制浓度(IC₅₀)为0.054 mg/mL,低于阿卡波糖、CPP、CPP+硒酵母、CPP+亚硒酸钠复配物。适宜浓度的Se-CPP可促进胰岛素抵抗状态HepG2细胞对葡萄糖的消耗,效果显著优于CPP、CPP+硒酵母、CPP+亚硒酸钠复配物(p<0.05)。

Abstract: The α-glucosidase and HepG2 cells were used to study the hypoglycemic activity of selenium polysaccharide from Cyclocarya paliurus(Batal)Ijinskaja(Se-CPP)in vitro. The hypoglycemic activity of Se-CPP was compared with Cyclocarya paliurus(Batal)Ijinskaja polysaccharide(CPP),CPP+selenium yeast and CPP+sodium selenite. The results showed that appropriate dose of Se-CPP could effectively inhibite the activity of α-glucosidase in a dose dependent and its 50% inhibitory concentration was 0.054 mg/mL,which was lower than acarbose,CPP,CPP+selenium yeast and CPP+sodium selenite. Furthermore,appropriate dose of Se-CPP could improve the glucose consumption of insulin resistance HepG2 cells and the effect was better than that of CPP,CPP+selenium yeast and CPP+sodium selenite(p<0.05).