Abstract: Lentinus edodes is a main medicinal and edible fungus,which is produced mainly through the cultivation of sawdust medium. We compared the differences in the content and molecular weight of lentinan for different batches,and analyzed their antioxidant capacity. The results showed that the lentinan of the first batch had the highest content and molecular weight,with 5.46% and 1018 kDa respectively,while the third batch had the lowest. In the antioxidant capacity,lentinan of different batches showed different antioxidant capacity. The DPPH free radical scavenging ability of the first batch was the strongest,and the hydroxyl radical scavenging ability of the second batch was the strongest,while the Fe³⁺ reducing power and total antioxidant capacity of the third batch was slightly higher than that of the first and second batch. This paper would provide a theoretical basis for the development and application of lentinan in different batches.