Abstract: Objective:To evaluate the immunomodulation effect of ten Lactobacillus strains in vitro and to explore the application of mouse spleen lymphocytes and macrophage in the selection of lactic acid bacteria with immunological activity. Methods:CCK-8 method was used to observe the effects of different doses of ten strains of Lactobacillus on the proliferation of mouse spleen lymphocytes and the level of energy metabolism, the effects of different doses of ten Lactobacillus strains on phagocytosis of macrophages were evaluated by the method of macrophage phagocytosis of neutral red. Results:The effect of ten strains of Lactobacillus on the proliferation of splenic lymphocytes and the phagocytosis of macrophages as well as the energy metabolism of macrophages showed a dose-effect relationship, among them, Lactobacillus plantarum KLDS 1.0318 had a higher regulation activity on spleen lymphocytes and macrophages. Conclusion:As immune cells, the mouse spleen lymphocytes and abdominal macrophages in vitro can be used to screen the Lactobacillus with potential immune activity. The effect of ten Lactobacillus strains on the activity of immune cells was evaluated in vitro, and the results showed that Lactobacillus plantarum KLDS 1.0318 had a higher immunomodulatory activity.