一株塔宾曲霉的分离鉴定及其在地沟油鉴别中的应用

文留坤; 缪福俊; 王云松; 祁世华; 邹子迪; 熊智

doi:10.13386/j.issn1002-0306.2018.01.023

一株塔宾曲霉的分离鉴定及其在地沟油鉴别中的应用

Isolation and identification of Aspergillus tubingensis and its application in the identification of gutter oil

摘要

摘要: 为了研究微生物在地沟油鉴别领域的应用,采用地沟油精炼技术对地沟油进行精炼,并从地沟油中分离获得8株真菌,其中发现菌株DGY03能够在地沟油培养基上快速生长繁殖,对其进行形态学观察以及采用分子生物学等方法进行鉴定。同时进一步探讨了菌株DGY03应用于地沟油鉴别领域的可行性,观察并比较其在地沟油和四种食用油培养基上的菌落生长速度、菌落长势以及培养基颜色变化等特征。结果表明,该菌株的菌落形态和显微结构与曲霉属基本相同,其ITS序列与Aspergillus tubingensis的同源性达100%,确定该菌株为塔宾曲霉(Genbank登录号:MF186869)。菌株DGY03在地沟油培养基上的菌落生长速度和菌落长势明显优于其它四种食用油培养基,其在地沟油培养基上的菌落生长速度为0.64 cm/d,而在大豆油、菜籽油、玉米油、花生油培养基上的菌落生长速度分别为0.21、0.28、0.24、0.29 cm/d。由此初步推断,利用菌株DGY03作为指示菌鉴别地沟油是可行的。

Abstract: To study the application of microorganisms in the field of gutter oil's identification. It used gutter oil refining technology to refine gutter oil. Eight strains of fungus were isolated from gutter oil, which showed that the strain DGY03 in gutter oil medium grew quickly, and it used the methods of morphological observation and molecular biology to identify it. It had studied its feasibility in the field of gutter oil's identify at the same time. The growth rate of and colony growth of the strain DGY03 in gutter oil and four kinds of edible oil medium were observed and compared. The results showed that the strain of colony morphology and microstructure were basically the same as Aspergillus, and the ITS sequence showed 100% homogeneous to Aspergillus tubingensis. So the strain DGY03 was determined to be Aspergillus tubingensis(The Genbank accession number:MF186869). And the growth rate and colony growth of strain DGY03 were significantly better than that of the other four edible oil media. The growth rate of the strain DGY03 on the gutter oil medium was 0.64cm/d, and the growth rate of soybean oil, rapeseed oil, corn oil and peanut oil medium were 0.21, 0.28, 0.24, 0.29 cm/d. Thus it could preliminary inference that it was feasible to use the strain DGY03 as indicator bacteria to identify gutter oil.