黑枸杞提取物对HBV的抑制作用及抗氧化能力测定

高恩怡; 龚红菲; 张艳群; 曾永联; 杨扬; 梁成钦; 韦京辰

doi:10.13386/j.issn1002-0306.2018.01.006

黑枸杞提取物对HBV的抑制作用及抗氧化能力测定

The inhibitory effect of black wolfberry extracts on HBV and the capacity of its antioxidant

摘要

摘要: 目的:研究黑枸杞提取物对抗乙肝病毒的作用并检测其抗氧化活性。方法:体外培养HepG22.2.15细胞,MTT法检测黑枸杞提取物对HepG22.2.15细胞的细胞毒作用,双抗体夹心法检测HepG22.2.15细胞培养液中HBsAg和HBeAg的表达。测定黑枸杞不同浓度提取物的还原能力和对羟自由基、DPPH自由基的清除能力。结果:MTT法检测黑枸杞提取物对HepG22.2.15细胞无明显细胞毒作用。与阴性对照组比较,黑枸杞提取物对HepG22.2.15细胞HBsAg和HBeAg的表达有抑制作用(p<0.05)。且随着黑枸杞提取物浓度的增加,其还原能力与清除羟自由基、DPPH自由基的能力逐渐增强。结论:黑枸杞提取物对乙肝病毒有抑制作用,并且有着良好的抗氧化能力。

Abstract: Objective:To study the effect of black wolfberry extracts against hepatitis B virus and to detect its antioxidant activity. Methods:HepG22.2.15 cells were cultured in vitro, MTT was used to detect the cytotoxicity of black wolfberry extracts against HepG2 2.2.15 cells. The expression of HBsAg and HBeAg in the culture medium of HepG2 2.2.15 cells was detected by double antibody sandwich method. To determine the reducing prower and scavenging capacity of hydroxyl radical and DPPH free radical of different concentration of black wolfberry.Results:MTT assay showed no obvious cytotoxicity of black wolfberry extracts on HepG2 2.2.15 cells. Compared with the negative control group, black wolfberry extracts had inhibitory effects on the expression of HBsAg and HBeAg in HepG2 2.2.15 cells(p<0.05).And with the increase of the concentration of black wolfberry extracts, its abilities of reducing and scavenging hydroxyl radical, scavenging DPPH free radical(DPPH·)were also gradually enhanced.Conclusion:black wolfberry extracts have inhibitory effect on hepatitis B virus, and have a good antioxidant capacity.