Abstract: In order to study the conditions of solid carrageenase preparation by spray drying, the conditions were determined by single factor test with the recovery of carrageenase activity as the evaluation index. Then the optimum conditions were determined by the response surface analysis method on the basis of the results of single factor experiment.The optimized spray drying parameters were shown as follows: maltodextrin 27%, inlet air temperature 131 ℃, hot air flow rate 4.5 m³/min, feeding speed 456 m L/h, atomizer pressure 0.2 MPa.Under the optimal process condition, the enzyme recovery of carrageenase reached 56.9% ± 2.1%, which was close to the theoretically predicted value of 56.1%.