Abstract: The enzymatic properties of recombined laccase derived from Pleurotus eryngii were investigated. The degradation effects of laccase on two kinds of phenols ( nonylphenol and 2, 4-dichlorophen) were evaluated through optimizing the degradation time, enzyme dosage, phenol concentration and pH. The optimal reaction temperature of recombined laccase was60 ℃, while the optimal pH was 2.5³.0.The recombined laccase could keep high thermal stability below 30 ℃ and high pH stability between 9.0¹0.0.The recombined laccase could decompose phenols with different structures, but the degradation ratios of phenols by laccase were decreased with increasing concentrations of phenols.With degradation time of of 16 h, enzyme dosage of 1 U/m L, pH of 3.5, the degradation ratio of nonylphenol ( 10 mg/L) reached up to 100%; with degradation time of 16 h, enzyme dosage of 1.25 U/m L, pH of 4.0, the degradation ratio of 2, 4-dichlorophen ( 5 mg/L) reached up to 95.1%.The study of recombined laccase derived from Pleurotus eryngii indicated a theoretical basis for the treatment of phenol-containing pollution.