牛初乳冻干粉和常乳冻干粉的降血糖及抗氧化作用

王小军; 高睿嘉; 郁桦; 狄怡琳; 李玲; 王艺茗; 丁宁; 魏扬智; 于天阳; 葛文金; 王荣昌; 程光宇

doi:10.13386/j.issn1002-0306.2017.23.048

牛初乳冻干粉和常乳冻干粉的降血糖及抗氧化作用

Hypoglycemic and antioxidation effect of lyophilized bovine colostrums and lyophilized milk

摘要

摘要: 目的:研究牛初乳冻干粉（LBC）和常乳冻干粉（LM）降血糖及抗氧化作用。方法:采用生化分析方法测定自由基清除活性及IGFs和胰岛素含量。建立小鼠四氧嘧啶糖尿病模型,分别给予降糖灵（100 mg/kg）、LM（600 mg/kg）和LBC（100 mg/kg、600 mg/kg）,以正常小鼠作对照。30 d后,测定血糖、糖耐量及抗氧化等指标。结果:LBC清除O₂^-·,DPPH·的EC₅₀值为245.3,167.2μg/m L,IGF-1、IGF-Ⅱ和胰岛素含量分别为1146.5、1303.0、128.4 ng/g。LM对羟自由基有一定清除作用,IGFs含量显著低于LBC,未检出胰岛素。LBC对糖尿病小鼠病症有明显改善;能显著降低血糖水平,降低血清MDA和TC含量;能提高糖耐量和血液SOD活性。LM对血糖水平和抗氧化指标无明显改善。结论:LBC比LM具有更高的降糖活性和自由基清除活性,对糖尿病小鼠有降血糖及抗氧化作用。

Abstract: Objective:To study on hypoglycemic effect and antioxidative effects of lyophilized bovine colostrums (LBC) and lyophilized milk (LM) .Methods:The free radical scavenging activity, IGFs and insulin content were detected by biochemical analysis.Alloxan diabetes model was induced in mice where rats were treated with Phenformin (100 mg/kg) , LM (600 mg/kg) and LBC (100、600 mg/kg) respectively.Besides, normal mice were used as control.The blood glucose, glucose tolerance and antioxidant and other indicators were measured after 30 days.Results:The EC₅₀of scavenging superoxide free radicals and DPPH free radicals were respectively 245.3, 167.2μg/m L in LBC.The content of IGF-I, IGF-Ⅱand insulin of LBC were 1146.5, 1303.0, 128.4 ng/g respectively.LM had a part of scavenging effect on hydroxyl free radicals, and the content of IGFs was significantly lower than LBC and insulin was not detected.LBC made significantly improvement on diabetic mice, significantly lower blood glucose levels, reduced the content of MDA and TC in serum, enhanced the tolerance and blood SOD activity.LM had no significant improvement in blood glucose level and antioxidant index.Conclusion:LBC included higher hypoglycemic factors, producted exhibited strong radical-scavenging activity in vivo than LM, which has hypoglycemic and antioxidative effects on diabetic mice.