Abstract: This article focused on the technique using GC-MS to determine the presence and concentrations of 45 kinds of organophosphorous pesticides in cider. 45 pesticides were tested with the recovery experiment of cider at three levels: 1, 5, 10 μg/L. The results showed that the 45 pesticides gave satisfactory recoveries ranging from 71.16% to 117.94% with the RSDs from 1.20% to 15.13% ( n = 5) , the limitation of this method was between 0.01 μg/L and 0.22 μg/L.45 pesticides were well separated and the linear relationship was good enough which the correlation coefficients were greater than 0.984 for concentrations from 10 to 2000 μg/L for most pesticides. Compared with conventional techniques, it was more accurate, lowcost, highly efficient and environmental friendly. Furthermore, for the limited use of organic solution, it has little harm to environment and human's health for the limited use of organic solution during the analysis process.