Abstract: A multiresidue method based on ultra performance liquid chromatography tandem mass spectrometry has been developed for the determination of twenty-seven β-agonists ( pyrbuterol, procaterol, reproterol, clenproperol, olodaterol. et al) residues in goat milk and powder.In this method, analytes were extracted by acidified acetonitrile, and purified by Oasis PRi ME HLB SPE column.twenty-seven β-agonists were analyzed by Acquity UPLC BEH C18 column ( 100 mm × 2.1 mm, 1.7 μm) using methanol and 0.1% formic acid as the mobile phases.The signals are acquired through the multi-channel MRM mode.All analytes could be well-separated by a gradient program during 10.5 minutes.The calibration curves of twenty-seven β-agonists were linear in a concentration range of 1.0¹00.0 μg/L ( r > 0.993) , and the limits of quantitation were all less than 0.5 μg/kg in goat milk.The recoveries of 0.5, 2.5, 5.0 μg/kg fortified samples ranged from 65.7% ¹19%, with the intra-assay coefficient of variation was 0.79% ¹0.4% and the inter-assay coefficient of variation was 2.13% ¹5.7%.The limits of quantitation were all less than 2.0 μg/kg in milk powder.The recoveries of 2.0, 10.0, 20.0 μg/kg fortified samples ranged from 60.5% ¹09%, with the intra-assay coefficient of variation was 1.91% ¹2.4% and the inter-assay coefficient of variation was 3.06% ¹7.2%.The method was established as a high-throughput method for confirmation of β-agonists residues in goat milk and powder.