超声辅助提取金针菇中麦角甾醇及其HPLC测定方法

胡代花; 张嘉昕; 李翠丽; 陈旺; 赵浩名; 王文博

doi:10.13386/j.issn1002-0306.2017.23.036

超声辅助提取金针菇中麦角甾醇及其HPLC测定方法

Optimization of ultrasonic-assisted extraction process of ergosterol from Flammulina velutipes and its determination by HPLC

摘要

摘要: 采用超声辅助提取方法,通过单因素实验和正交实验,对影响麦角甾醇提取效果的主要因素提取溶剂、提取时间、料液比和提取温度进行研究,并建立金针菇中麦角甾醇HPLC测定方法。结果表明,各因素对金针菇中麦角甾醇得率的影响程度依次为提取次数、料液比、提取温度和提取时间。最佳提取条件为无水乙醇超声提取2次,每次20 min,料液比1∶50,提取温度50℃,在该提取条件下,麦角甾醇得率高达2.28 mg·g^-1。麦角甾醇的线性范围为10.00¹00.00μg·m L^-1,相关系数为0.9998,平均回收率（n=9）为100.31%,RSD（n=9）为2.69%,分析周期11 min。该法与传统皂化回流提取相比,提取效率及重现性均较好。

Abstract: The main factors affecting the ultrasonic-assisted extraction efficiency of solvent types, times, ratios of solvent to material, duration and temperature on the yield of ergosterol in Flammulina velutipes were researched respectively by singlefactor experiments, and the extraction process were optimized by orthogonal tests.The results showed that the factors affecting the extraction rate of ergosterol were in the order of extraction duration, extraction ratios of solvent to material, extraction times and extraction temperature.The optimum extraction conditions were as follows:the extraction time 20 min and number of extraction 2 times, the ratio of material to ethanol was 1∶50 (g/m L) , extraction temperature 50℃.Under these conditions, the average extraction yield of ergosterol was 2.28 mg·g^-1.The linear range of ergosterol was 10.00¹00.00μg·m L^-1 (R²=0.9998) , the average recoveries (n=9) was 100.31%with RSD 2.69%, and the analysis cycle was within 11 minutes.This method showed significantly higher extraction yield and reproducibility compared with conventional reflux.