Abstract: Polysaccharides exhibite immune activity and regulate gut microbiota. Gut microbiota can play an important role in metabolism of polysaccharides and immune function of human hosts. At the same time, it is influenced by the immune. In this paper, the related progress in interaction of polysaccharides, gut microbiota and immunity was summarized, which provided guidelines to explore the intrinsic relationship and action mechanism among polysaccharides, gut microbiota and immunity.