响应面法优化薏米中β-谷甾醇的提取工艺
Optimization of extraction technology of β-sitosterol from coix seed by response surface methodology
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摘要: 目的:采用超声波辅助提取薏米中的β-谷甾醇,为其在功能性食品等加工利用方面提供参考。方法:以薏米为原料,磨碎成粉,以乙醇为提取溶剂,以液料比、超声温度、超声时间、乙醇浓度为单因素,以β-谷甾醇提取得率为指标,运用响应面法研究各因素及其交互作用对薏米中β-谷甾醇提取得率的影响。结果:超声辅助法提取薏米中的β-谷甾醇最佳提取条件:液料比17.5∶1 m L/g、超声温度52℃、超声时间38 min。在此条件下,以95%乙醇为提取溶剂,β-谷甾醇平均提取得率为8.93 mg/g,接近模型中的预测值8.97 mg/g。结论:该方法操作简便,得率较高,具有实际意义。Abstract: Objective: Extraction of β-sitosterol from coix seed by ultrasound, and use to provide a reference for processing and utilization of functional food.Methods: In this experiment, coix seed was used as raw material and ground into powder, and the ratio of material to liquid, ultrasonic temperature, ultrasonic time and ethanol concentration were taken as single factor. The response surface method was used to study on the effects of different factors and their interactions on β-sitosterol extraction rate in coix seed.Results: The optimal extraction conditions of β-sitosterol in coix seed were as follows: liquid ratio ( methanol: coix seed) was 17.5∶ 1 m L/g, ultrasonic temperature was 52 ℃, ultrasonic time was 38 min.Under these conditions, the β-sitosterol average extraction rate was 8.93 mg/g close to the predicted value 8.97 mg/g in the model. Conclusion: The method had the advantages of simple operation, high yield and practical significance.
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