化学添加剂对裂壶藻突变株发酵产DHA的影响

林源锋; 谢鑫磊; 付杰; 田华; 陈涛; 何东平

doi:10.13386/j.issn1002-0306.2017.22.025

化学添加剂对裂壶藻突变株发酵产DHA的影响

Effect of chemical modulators on DHA production by fermentation of Schizochytrium limacinum mutant strain

摘要

摘要: 为了研究外源化学添加剂对裂壶藻（Schizochytrium limacinum）突变株发酵合成脂肪酸（SFAs、PUFAs和DHA）的影响,采用基于气相色谱-质谱（GC-MS）联用技术分析裂壶藻突变株产油脂肪酸的组成。通过单因素和正交实验设计,优化了乙醇胺（ETA）、萘氧乙酸（BNOA）和水杨酸（SA）三种化学添加剂的最佳添加条件。结果表明,添加150 mg/L的乙醇胺（ETA）、10 mg/L萘氧乙酸（BNOA）和1 mg/L水杨酸（SA）,裂壶藻突变株的DHA产量最高,达到6.18 g/L,比对照组提高了12.77%。综上所述,添加适量的化学添加剂可以提高裂壶藻突变株DHA的含量。

Abstract: In order to investigate the effects of chemical modulators on the synthesis of fatty acids ( SFAs, PUFAs and DHA) in Schizochytrium limacinum mutant strain, the composition of fatty acids in Schizochytrium limacinum mutant strain was analyzed by chromatography-mass spectrometry ( GC-MS) .The optimum addition conditions of three chemical modulators, ethanolamine ( ETA) , naphthalene acetic acid ( BNOA) and salicylic acid ( SA) were optimized by single factor and orthogonal design.The results showed that the adding ethanol amine 150 mg/L ( ETA) , 10 mg/L naphthoxyacetic acid ( BNOA) and 1 mg/L salicylic acid ( SA) , the DHA production of Schizochytrium limacinum mutant strain was the highest, reaching 6.18 g/L, which was12.77% higher than control group.It was suggested that the adding appropriate amount of chemical modulators could improve DHA content of Schizochytrium limacinum mutant strain.