Abstract: Wild yeast strains are important resources for brewing wine.In this study, in order to find valuable wild yeast strains, streak plate separation procedures were used and three strains, YYMPT-1, YYMPT-2 and YYMPT-3, were obtained using Xiahei grape as object. They were identified as Hanseniaspora uvarum, Sporidiobolus pararoseus and Candida zemplinina by amplifying D1/D2 domain of 26 S ribosomal gene ( 26 S r DNA) , internal transcribed spacer sequence ( ITS1-5.8 S-ITS2) and actin gene by polymerase chain reaction ( PCR) , and established their phylogenetic tree, as well as observed their morphological features by scanning electron microscopy ( SEM) . The results from the current research provided 3 potential strains used for industrial applications for the production and improvement of wine quality with desirable flavor.