番木瓜叶不同溶剂提取物的抗氧化性能

刘玉革; 付琼; 马飞跃; 张秀梅

doi:10.13386/j.issn1002-0306.2017.21.003

番木瓜叶不同溶剂提取物的抗氧化性能

Antioxidant activities of papaya leaf extracts with different extraction solvents

摘要

摘要: 以番木瓜叶为研究对象,用水、70%甲醇和70%乙醇对其中活性物质进行了提取。测定了三种试剂提取率、总酚以及总黄酮含量,还对提取物的自由基清除能力、金属离子螯合能力（MCC）和铁离子氧化还原能力（FRAP）进行了研究。结果表明提取得率和总酚含量的大小顺序是:水>70%甲醇>70%乙醇,而总黄酮的含量与此相反。三种提取物都具有良好的DPPH·、ABTS⁺·和·OH自由基清除能力、金属离子螯合能力（MCC）和铁离子氧化/还原能力（FRAP）。综合考虑提取率、活性物质含量和各种抗氧化指标,70%甲醇被选为最合适的番木瓜叶提取试剂。该研究为番木瓜叶在制备天然抗氧化剂方面的应用提供了实验基础,还提高了番木瓜的整体利用价值。

Abstract: The bioactive compounds of payapa leaf were extracted by water, 70% methanol and 70% ethanol. The extraction efficiency, contents of total phenol and flavonoid were determined. The scavenging abilities of free radicals, metal chelating capacity ( MCC) and ferric reducing antioxidant power ( FRAP) of the three extracts were also investigated. Results showed that the order of extraction efficiency and total phenol content was as follows: water > 70% methanol > 70% ethanol, while the order of total flavonoid was the opposite.The three extracts obtained by three solvents all possessed excellent DPPH·, ABTS⁺·and·OH radicals scavenging abilities, MCC and FRAP.70% methanol was chosen as the most appropriate solvent for the extraction of papaya leaf.The research here not only provided experimental basis for the usage of papaya leaf in the synthesis of natural antioxidants, but also improved the overall value of papaya.