Abstract: In this paper, p H and protein of fresh eggs were detected by visible/near infrared spectroscopy technology.The diffuse reflectance spectra of fresh eggs in 400 ~ 1000 nm and 900 ~ 1700 nm were collected respectively. The optimal pretreatment method was selected by spectral preprocessing techniques such as multiple scattering correction ( MSC) and standard normal variable transform ( SNV) .Partial least squares ( PLS) was used to establish p H-protein and protein model then evaluated it.The results showed that the p H model based on the spectral range of 900 ~ 1700 nm was better, the correlation coefficient of the calibration set was 0.948, the correlation coefficient was 0.855.The protein model based on the spectral range of 400¹000 nm was better, the correlation coefficient of the calibration set was 0.927, and the correlation coefficient of the prediction set was0.906. The research showed that visible/near infrared spectroscopy could rightly predict the p H and protein of fresh eggs, and it could provide new ideas and methods for rapid nondestructive testing of nutrient components.