Abstract: Yield of Osmanthus essential oil was to measure the improvement of Osmanthus essential oil extraction. In a single factor such as temperature, time, dosage, liquid ratio, ratio of β-glucosidase, and p H on the effect of the yield of essential oil, the response surface analysis was used to optimize the enzyme treatment conditions and the establishment of a reliable multiple quadratic regression model.The results showed that when the liquid to solid ratio of 20.3, enzyme dosage 50.7 IU/g including48.3% β-glucosidase, in 46 ℃, p H4.6 under reflux reaction 2.7 h, yield of Osmanthus essential oil could be raised from 1.77%to 2.75%, which was raised 55.35%.Compared with only β-glucosidase used, the yield was raised 1.30 times. L-linalool, β-ionone, dihydro-β-ionone, β-ionol, γ-decalactone, geraniol, perilla alcohol and nerol of the essential oil was increased by56.33%, 4.38%, 21.54%, 169.07%, 56.52%, 230.92%, 157.47%, 154.41% and 16.97% in the content of phthalate esters by GC-MS. The yield of enzyme-assisted extracting Osmanthus essential oil was improved while the content of main aroma components in Osmanthus oil could be increased and the content of harmful substances also could be reduced.Thus, the quality of the Osmanthus oil was improved.